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发明公开
CN107034236A 利用杆状病毒表达系统制备猪流行腹泻病毒S1蛋白的方法 无效 - 驳回

利用杆状病毒表达系统制备猪流行腹泻病毒S1蛋白的方法
摘要：
本发明属于微生物技术领域，旨在提供一种利用杆状病毒表达系统制备猪流行腹泻病毒S1蛋白的方法。包括以下步骤：PEDV的S1基因克隆和密码子的优化；构建PEDV‑S1基因重组杆状病毒表达载体；表达重组PEDV‑S1蛋白的杆状病毒包装和传代；优化表达重组PEDV‑S1蛋白的病毒在sf9细胞表达条件；纯化重组PEDV‑S1蛋白。本发明安全性较高，不存在PEDV弱毒返强的风险；具有天然构象，具有较好的模拟PEDV病毒的免疫原作用；高滴度的免疫原，方便注射或者口服给药。
摘要（英）：

The invention belongs to the technical field of microbiology and is intended to provide a method for preparing porcine epizootic diarrhea virus S1 protein by using a baculovirus expression system; the method comprises the steps of cloning PEDV (porcine epizootic diarrhea virus) S1 gene, and optimizing a codon; constructing a PEDV-S1 gene recombinant baculovirus expression vector; performing packaging and passage on baculovirus for expressing recombinant PEDV-S1 protein; optimizing expression conditions of the virus for expressing the recombinant PEDV-S1 protein in sf9 cells; purifying the recombinant PEDV-S1 protein. The method has the advantages that the safety is high, the method has no risk of reversion of PEDV; native conformation is provided, and good immunogen action to simulate PEDV is achieved; immunogens of high titer facilitate the injection or oral administration of drugs.

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