The invention discloses a time slot partition method for a power utility information acquisition system based on power line communication. The method comprises the following steps: 1, partitioning time slots into a beacon time slot, a routine service time slot, a burst service time slot and a silent time slot according to the types of transmitted services; and 2, adopting CSMA/CA (Carrier Sense Multiple Access/Collision Avoidance) or a centralized control TDMA (Time Division Multiple Address) as a channel access way in each type of time slot. Through adoption of the time slot partition method, the technical problem that the probability that a burst service is preferentially served cannot be ensured in a priority control way along with the increase of the routine service communication data amount in the power utility information acquisition system based on power line communication in the prior art is solved.