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发明公开
CN107056414A 一种广谱性热带兰无菌播种培养基二 无效 - 驳回

一种广谱性热带兰无菌播种培养基二
摘要：
本发明公开了一种广谱性热带兰无菌播种培养基二，每L培养基中包括：NH4NO3 120g、KNO3 34g、CaCl2·2H2O 440g、MgSO4·7H2O 370g、KH2PO4 90g、蔗糖20g、活性炭2g、琼脂5.8g，其余为1/4MS的微量元素、有机成分和铁盐，pH＝5.7。采取成熟果荚时期进行培养：采取未开裂果荚，果荚果柄部位开始变黄时采取。培养温度24至26℃，光照强度为60μmol·m‑2s‑1，光照时间12小时/天。使用该培养基，兜兰属种子萌发率在70％以上，卡特兰属、蝴蝶兰属、石斛兰属种子萌发率在90％以上，其他热带兰播种种子萌发率也都在80％以上。
摘要（英）：

The invention discloses a wide-spectrum sterile sowing culture medium II for tropical orchids. Each L of culture medium contains the following components by weight: 120g of NH4O3, 34g of KNO3, 440g of CaCl2.2H2O, 370g of MgSO4.7H2O, 90g of KH2PO4, 20g of saccharose, 2g of activated carbon, 5.8g of agar and the balance of 1/4MS microelements, organic components and iron salt; and the pH value of the culture medium is 5.7. The tropical orchids are cultured by the following steps: carrying culturing in a mature fruit pod period; and picking un-cracked fruit pods when fruit stems of the fruit pods starting to yellow, wherein the culture temperature is 24-26 DEG C, the illumination intensity is 60 micro mol.m/2s<-1>, and the illumination time is 12 hour/day. By utilizing the culture medium, the germination rate of paphiopedilum seeds is above 70%, the germination rates of cattleya, butterfly orchid and dendrobium seeds are above 90%, and the germination rates of other tropical orchid seeds are above 80%.

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