The invention provides a negative voltage generation circuit with an automatic voltage regulation function. The negative voltage generation circuit comprises a negative voltage generation circuit and a feedback control module; and the feedback control module performs regulation on the negative voltage generated by the negative voltage generation circuit. According to the negative voltage generation circuit with the automatic voltage regulation function provided by the invention, the size of the charging current of a charge pump can be automatically regulated according to the size of the load current so as to realize stability of the output voltage; consequently, a conventional analog circuit structure still can work as normal in an extremely low power supply voltage, so that the negative voltage generation circuit is particularly suitable for a deep submicron process; and meanwhile, digital adjustability of the output voltage is also realized, without outputting single negative electrode, and the negative voltage value needing to be output can be adjusted according to actual requirements.