The invention provides a livestock and poultry manure fermentation microbial agent and a method for applying the same, and belongs to the technical field of microbial fermentation. The livestock and poultry manure fermentation microbial agent comprises zymocyte and biological active factors. A mass ratio of the zymocyte to the biological active factors is 100:2-8. The method for applying the livestock and poultry manure fermentation microbial agent includes adding the livestock and poultry manure fermentation microbial agent into livestock and poultry manure with the water content of 50-60 wt% according to a mass ratio of 0.05-5:1000; mixing the livestock and poultry manure fermentation microbial agent and the livestock and poultry manure with each other and then piling the livestock and poultry manure fermentation microbial agent and the livestock and poultry manure to obtain piles; turning the piles at the intervals of 16-32 h when the temperatures of the piles reach 40-45 DEG C and keep unchanged; piling the piles to obtain bins after the temperatures of the piles reach 60-70 DEG C and then keep unchanged for 3-5 d and the water contents of the piles are lower than 30 wt%, acquiring fermentation products when white hyphae appear on the surfaces of the bins. The livestock and poultry manure fermentation microbial agent and the method have the advantages that the livestock and poultry manure can quickly start to be fermented, and smell generated by the livestock and poultry manure in fermentation procedures can be reduced.