The invention discloses an anti-bird needle plate of power transmission line and the application method thereof. The anti-bird needle plate comprises a lower insulating plate and an upper insulating plate, wherein the two insulating plate are oppositely bonded together. The corresponding part of the upper surface of the lower insulating plate and the corresponding part of the lower surface of the upper insulating plate are provided with semi-circular grooves, and a circular hole is formed by the two semi-circular grooves. A rotating shaft is arranged in the circular hole. The rotating shaft is provided with a plurality of through holes, and anti-bird needles are penetrated in the through holes. The upper insulating plate is provided with a corner-shaped groove. The anti-bird needles penetrate out of the corner-shaped groove of the upper insulating plate. The bottom of the lower insulating plate is provided with a base. The anti-bird needle plate is convenient to assemble and disassemble. The number of anti-bird needles can be set according to the size of tower materials, and the anti-bird needle plate is obvious in anti-bird effect. Meanwhile, anti-bird needles can be rotated, so that the development of the maintenance operation of the line is not affected at all.