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发明授权
CN107129936B 一种产紫杉醇的青霉BP6T3及其应用 有权

一种产紫杉醇的青霉BP6T3及其应用
摘要：
本发明公开了一株从红豆杉科巴山榧树的组织中分离筛选出的产紫杉醇的内生菌，经过分类鉴定命名为青霉(Penicillium sp.)BP6T3，保藏编号为CCTCC NO:M2016320。该菌株具有抗金黄色葡萄球菌和大肠杆菌的活性，发酵液中脂溶性提取物含量可达105mg/L，其中紫杉醇的相对百分含量为15.8%，产量为16.59 mg/L，高于现有技术已公开的产量；以L‑苯丙氨酸为前体，紫杉醇的产量可增长425.17%。采用CCK‑8试剂法证明了青霉BP6T3的发酵液提取物对Hela肿瘤细胞的抑制作用，抑制率可达到56.68%。
摘要（英）：

The invention discloses a paclitaxel-producing endophyte separated and screened from tissues of Taxaceae Torreya fargesii Franch.. The paclitaxel-producing endophyte is classified and identified, and is named as Penicillium sp. BP6T3, and the preservation number of the Penicillium sp. BP6T3 is CCTCC NO:M2016320. The above strain has anti-Staphylococcus aureus and anti-Escherichia coli activity, the content of fat-soluble extract in a fermentation solution can reach 105 mg/, the relative percentage of paclitaxel is 15.8%, and the yield is 16.59 mg/L and is higher than yields disclosed in the prior art; and the yield of the paclitaxel can be increased by 425.17% when L-phenylalanine is used as a precursor. A CCK-8 reagent method is adopted to prove that the extract in the fermentation solution of the Penicillium BP6T3 has an inhibition effect on Hela tumor cells, and the inhibition rate can reach 56.68%.

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