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CN107142280A 一种表达H9亚型禽流感病毒HA基因的重组火鸡疱疹病毒株 无效 - 驳回

一种表达H9亚型禽流感病毒HA基因的重组火鸡疱疹病毒株
摘要：
本发明提供一种表达H9亚型禽流感病毒HA基因重组火鸡疱疹病毒株，本发明所提供的表达H9亚型禽流感病毒HA基因重组火鸡疱疹病毒(rHVT‑H9‑HA)株，于2017年1月18日保藏在位于中国武汉、武汉大学的中国典型培养物保藏中心，保藏编号为CCTCC NO:V201707。本发明以火鸡疱疹病毒FC‑126疫苗毒株为载体，分别以其复制非必需区US2和US10为插入位点，构建表达EGFP标记基因的重组病毒rHVT‑EGFP。本发明选择H9亚型禽流感病毒YZ140706株的HA基因完整的开放阅读框和HA2基因分别构建表达盒，再次利用同源重组原理，用上述两个基因表达盒分别替换重组病毒rHVT‑EGFP的标记基因eGFP，分别筛选出表达完整HA基因和部分HA基因的重组HVT。
摘要（英）：

The invention provides a recombinant herpes turkey virus (HVT) strain for expressing a gene HA of an H9 subtype avian influenza virus. The recombinant herpes turkey virus (rHVT-H9-HA) strain for expressing the gene HA of the H9 subtype avian influenza virus, provided by the invention, is collected in CCTCC of Wuhan University, Wuhan, China, at January 18, 2017 and has the collection number of CCTCC NO: V201707. According to the rHVT-H9-HA strain, a vaccine strain FC-126 of HVT serves as a vector and is used for separately copying nonessential regions US2 and US10 so as to obtain insertion sites and construct a recombinant virus rHVT-EGFP for expressing an EGFP marker gene. According to the rHVT-H9-HA strain, two expression kits are separately constructed by selecting a complete open reading frame of the gene HA of an H9 subtype avian influenza virus strain YZ140706 and a gene HA2, a marker gene eGFP of the recombinant virus rHVT-EGFP is separately replaced with the two gene expression kits by using a homologous recombination principle, and thus, recombinant HVTs for expressing a complete HA gene and a partial HA gene are separately screened out.

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