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发明公开
CN107151674A 新型抗菌肽DLP4的制备方法 无效 - 撤回

新型抗菌肽DLP4的制备方法
摘要：
本发明提供新型抗菌肽DLP4的制备方法，利用基因工程技术，通过对编码抗菌肽DLP4基因的优化，实现其在毕赤酵母中的重组表达。本发明首次实现抗菌肽DLP4在毕赤酵母基因工程菌中的表达，经测定发酵纯化产物对金黄色葡萄球菌ATCC 25923，ATCC 43300和ATCC 6538以及猪链球菌CVCC 606具有显著抑菌活性，MIC介于0.031‑2μg/ml。采用本发明方法制备的抗菌肽DLP4可应用于抗菌药物、食品添加剂、化妆品、饲料添加剂等领域，具有广阔的应用价值和市场前景。
摘要（英）：

The invention provides a preparation method of novel antibacterial peptide DLP4. Recombinant expression of the antibacterial peptide DLP4 in pichia pastoris is realized by using a genetic engineering technology and optimizing an encoded antibacterial peptide DLP4 gene. According to the preparation method disclosed by the invention, the expression of the antibacterial peptide DLP4 in recombinant pichia pastoris is realized for the first time; by measurement, a fermentation purified product has remarkable bacteriostatic activity for staphylococcus aurei ATCC25923, ATCC43300 and ATCC6538 as well as streptococcus suis CVCC606; MIC (Minimal Inhibitory Concentration) is 0.031 to 2mug/ml. The antibacterial peptide DLP4 prepared by adopting the method disclosed by the invention can be applied to the fields of antibacterial drugs, food additives, cosmetics, feed additives and the like and has high application value and a broad market prospect.

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