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发明公开
CN107164283A 一种L‑丙氨酸高密度菌液培养基及其培养方法 无效 - 驳回

一种L‑丙氨酸高密度菌液培养基及其培养方法
摘要：
本发明涉及发酵工程技术领域，具体公开一种L‑丙氨酸高密度菌液培养基及其培养方法。所述培养基包括第一原料和第二原料，所述第一原料包括如下重量份数的下列组分：甘油1‑10份，酵母粉10‑30份，大豆蛋白胨5‑15份，麦芽汁1‑10份，磷酸铵1‑5份，磷酸二氢钾1‑5份，三水磷酸氢二钾3‑15份，氯化钠1‑5份；所述第二原料包括20‑40ppm的六水氯化钴，2‑4ppm的一水硫酸锰，20‑40ppm的二水钼酸钠。经摇瓶培养，培养完毕后，得到种子液，经放大发酵处理得到L‑丙氨酸高密度菌液。本发明摇瓶种子OD600值增长84%，发酵周期缩短20h，L‑丙氨酸产量提高52%，降低生产成本。
摘要（英）：

The invention relates to the technical field of fermentation engineering, and in particular discloses an L-alanine high-density bacteria liquid culture medium and a cultivation method thereof. The L-alanine high-density bacteria liquid culture medium includes a first material and a second material, the first material comprises the following components in parts by weight: 1-10 parts of glycerol, 10-30 parts of yeast powder, 5-15 parts of soybean peptone, 1-10 parts of malt juice, 1-5 parts of ammonium phosphate, 1-5 parts of potassium dihydrogen phosphate, 3-15 parts of dipotassium hydrogen phosphate trihydrate and 1-5 parts of sodium chloride; the second material includes 20-40ppm of cobalt chloride hexahydrate, 2-4ppm of manganese sulfate monohydrate and 20-40ppm of sodium molybdate dehydrate. A seed liquid is obtained after completion of shake-flask culture, and an L-alanine high-density bacteria liquid is obtained by scale-up fermentation. The shake-flask seed OD600 value is increased by 84%, the fermentation cycle is shortened by 20h, the L-alanine production yield is increased by 52%, and the production cost is reduced.

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