利索-全球专利检索

  1. 登录
  2. 注册
发明公开
CN107164373A 大豆低温诱导人工合成启动子SP5及应用 失效 - 权利终止

大豆低温诱导人工合成启动子SP5及应用
摘要：
本发明公开了一种大豆低温诱导启动子SP5，它是以大豆中的Glyma18g03930基因启动子区 179 bp、Glyma09g29840基因启动子区382 bp、以及35S核心启动子Core CaMV 35S连接构建而成，命名为Synthetic Promoter 5，简称为SP5。在转基因烟草中鉴定SP5启动子驱动报告基因GUS的表达情况表明，SP5启动子可以受干旱、盐、盐碱、ABA以及低温胁迫所诱导，对低温尤其敏感。本发明构建了一种大豆人工合成启动子SP5，其对低温胁迫尤其敏感，可直接应用于农作物转基因抗逆育种。
摘要（英）：

The invention discloses a soybean low-temperature-induced promoter SP5. The promoter SP5 is constructed by connecting a Glyma18g03930 gene promoter region 179bp, a Glyma09g29840 gene promoter region 382bp and a 35S core promoter Core CaMV 35S, and is named as Synthetic Promoter 5, called SP5 for short. The method for identifying expression conditions of SP5 promoter-driven reporter genes GUS (Glucuronidase) in transgenic tobacco shows that the promoter SP5 can be induced by drought, salt, salt and alkali, ABA (Abscisic Acid) and low-temperature stress and is particularly sensitive to low temperature. The soybean artificial synthetic promoter SP5 constructed in the invention is particularly sensitive to low-temperature stress and can be directly applied to transgenic stress resistance breeding of crops.

公开/授权文献
0/0