The invention discloses a multi-AGV dispatching system based on a unidirectional and bidirectional mixed path. Each guide track is provided with two identification cards, each path node is provided with an identification card as an execution card, RFID readers installed on AGVs read identification card information and upload the information to a master control system, the positions of the AGVs are determined according to electronic tag information, then according to task allocation, an offline path is obtained through an A* algorithm and is sent to the AGVs, the positions of the AGCs are monitored on line in real time, and conflicts among the multiple AGVs are prevented through a point-side anti-conflict algorithm.