The invention relates to a cultivation method of a Jingshu No.6 virus-free seedling stock culture. The cultivation method comprises the following steps: selecting a seedbed as a seedbed or production field and carrying out soil treatment, then ridging and cultivating, thoroughly ploughing the center of a ridge, carrying out disinfection treatment on a Jingshu No.6 virus-free seedling before cultivation, cultivating and raising seedlings by adopting a horizontal planting method, horizontally transplanting and cutting a pretreated virus-free seedling into the seedbed, pouring rooting water, fertilizing, and harvesting the virus-free seedling stock culture. The cultivation method provided by the invention has the advantages that a cultivation environment of the Jingshu No.6 virus-free seedling stock culture is guaranteed by selecting the seedbed and carrying out the soil treatment and virus-free seedling pretreatment, a horizontal transplanting and cutting way is adopted, and the rooting water is poured, so that the survival rate of the Jingshu No.6 virus-free seedling stock culture in a cultivation process is improved, the growth vigor of the virus-free seedling stock culture is effectively enhanced by applying available nitrogen fertilizer, and the quality of the virus-free seedling stock culture is also improved.