The invention discloses a method for separating 2-naphthalene sulfonic acid in waste water by iron-copper supported macroporous resins, and relates to the technical field of separation of the 2-naphthalene sulfonic acid in the waste water. The method includes the steps: sufficiently mixing pretreated macroporous resins and a certain concentration of FeCl3 and CuCl2; adding a certain concentration of solution of NaCl and HCl; soaking mixture by the aid of solution of NaCl and ammonia water after oscillation for a certain time on a swing bed; washing the mixture to be colorless by the aid of absolute ethyl alcohol, and washing the mixture by the aid of deionized water until solution is neutral to obtain the iron-copper supported macroporous resins; sufficiently mixing a certain quantity of iron-copper supported macroporous resins and 2-naphthalene sulfonic acid waste water, enabling the resins and the waste water to be balanced in a constant-temperature water bath oscillator, flushing the resins by the aid of a certain concentration of alkali liquor after adsorption saturation of the resins, and performing elution and regenerated of the resins. The method has the advantages that the resins are high in adsorption capacity, high in selectivity, rapid in exchange and adsorption, easy to regenerate, long in service life and low in treatment cost, 2-naphthalene sulfonic acid in industrial wastewater can be effectively separated, and the regeneration rate of the resins reaches up to 95% or more.