The invention provides a kit for screening a blood type irregular antibody, and belongs to the technical field of blood type antibody detection. The kit contains six parts of erythrocytes which are packaged separately and divided into three groups; the three groups of erythrocytes come from three persons respectively; at least two of D, C, c, E, e, s, Le, Fy and K antigens in the erythrocytes of each person are positive, and at least one of M, N, S, Le, JK, JK, Fy and P1 antigens is positive; antigen components of the three persons are complementary; each group of erythrocytes consists of two parts of independent erythrocytes packaged in the same specification; one part of erythrocytes is treated with bromelain, and the other part of erythrocytes is not treated with the bromelain; the concentrations of the six parts of erythrocytes in the kit are the same, and can be 1 to 5 percent. The kit is suitable for various blood type antibody detection methods in the prior art. By adopting the kit, the omission ratio of irregular antibodies is reduced, and the clinical blood transfusion risk is lowered.