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发明公开
CN107216379A 半滑舌鳎IGF‑I蛋白及其体外表达制备方法与应用 无效 - 撤回

半滑舌鳎IGF‑I蛋白及其体外表达制备方法与应用
摘要：
本发明涉及一种半滑舌鳎IGF‑I蛋白及其体外表达制备方法与应用，所述的蛋白的核苷酸序列为SEQ IDNO:1；氨基酸序列为SEQ IDNO:3。本发明还提供一种半滑舌鳎IGF‑I蛋白的体外表达制备方法，它包括成熟肽序列改造、重组表达载体构建、重组表达载体诱导表达和重组蛋白纯化；本发明利用按照酵母密码子偏好性改造过的半滑舌鳎IGF‑I成熟肽序列与真核表达载体构建了重组表达载体，并在酵母工程菌中成功实现了IGF‑I的高效表达，获得了具有生物活性的半滑舌鳎IGF‑I重组蛋白，以饲料添加剂的形式应用后具有明显的促生长效果。
摘要（英）：

The invention relates to cynoglossus semilaevis IGF-I (Insulin-like Growth Factor-I) protein, and an in-vitro expression preparation method and application thereof. A nucleoside sequence of the cynoglossus semilaevis IGF-I protein is as shown in a SEQ ID NO:1, and an amino acid sequence is as shown in a SEQ ID NO:3. The invention also provides an in-vitro expression preparation method of the cynoglossus semilaevis IGF-I protein. The in-vitro expression preparation method comprises the steps of mature peptide sequence modification, recombinant expression vector construction, recombinant expression vector inducible expression and recombinant protein purification. According to the cynoglossus semilaevis IGF-I protein provided by the invention, a recombinant expression vector is constructed by utilizing a cynoglossus semilaevis IGF-I mature peptide sequence modified according to yeast codon preference and a eukaryotic expression vector, high-efficiency expression of IGF-I in yeast engineered bacteria is realized, cynoglossus semilaevis IGF-I recombinant protein with bioactivity is obtained, and an obvious growth promotion effect is obtained after applying the cynoglossus semilaevis IGF-I protein in a feed additive form.

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