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发明公开
CN107217078A 伯醇的氧化和胺化 无效 - 撤回 转让

伯醇的氧化和胺化
摘要：
本发明涉及伯醇的氧化和胺化。具体地，本发明包括下述步骤的方法：提供下式的伯醇HO‑(CH2)x‑R1，其中R1选自–OH、‑SH、‑NH2 和–COOR2，x是至少3且R2选自H、烷基和芳基，b) 通过与NAD(P)+依赖性的醇脱氢酶接触氧化所述伯醇，和c) 使步骤a) 的氧化产物与转氨酶接触，其中所述NAD(P)+依赖性的醇脱氢酶和/或所述转氨酶是重组的或分离的酶，涉及用于实行该方法的全细胞催化剂和这种全细胞催化剂用于氧化伯醇的用途。
摘要（英）：

The invention relates to oxidation and amination of primary alcohols. The invention relates to a method comprising the following steps: a) providing a primary alcohol of the formula HO-(CH2)x-R1, in which R1 is selected from the group comprising -OH, -SH, -NH2, and -COOR2, x is at least 3, and R2 is selected from the group comprising H, alkyl, and aryl, b) oxidising the primary alcohol by bringing it into contact with an NAD(P)+-dependent alcohol dehydrogenase, and c) bringing the oxidation product from step a) into contact with a transaminase, the NAD(P)+-alcohol dehydrogenase and/or the transaminase being a recombinant or an isolated enzyme. The invention also relates to a whole-cell catalyst for carrying out said method, and to the use of such a whole-cell catalyst for oxidising a primary alcohol.

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