The invention discloses bio-enzyme washing liquid for infant clothing, which comprises the following ingredients by weight percentage: 30-50% of non-ionic surfactant, 0.1-0.3% of chelating agent, 4.2-10.5% of solubilizer, 0.3-0.7% of preservative, 1-3% of defoaming agent, 1-5% of compound enzyme preparation and the balance of deionized water. The bio-enzyme washing liquid for the infant clothing adopts highly safe and easily degradable raw materials, is neutral, does not injure skin or damage the clothing, and kills and resists bacteria; no essence or pigment is added; the compound enzyme preparation and the non-ionic surfactant in the ingredients exert a synergistic effect; the liquid can quickly and efficiently remove stains such as proteins, fats, sugar, sweat and excrement on the infant clothing and a milk smell or a peculiar smell on the infant clothing; the infant clothing is easy to rinse after washing; chemical component residues are very few; and at the same time, the liquid has the characteristics of an excellent cleaning effect and high safety.