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发明公开
CN107298705A 半滑舌鳎MCH2蛋白及其体外表达制备方法和应用 无效 - 驳回

半滑舌鳎MCH2蛋白及其体外表达制备方法和应用
摘要：
本发明涉及一种半滑舌鳎MCH2蛋白及其体外表达制备方法和应用，所述蛋白的核苷酸序列为SEQ ID NO:1；所述蛋白氨基酸的序列为SEQ ID NO:2。所述半滑舌鳎MCH2蛋白的体外表达制备方法，它包括重组表达载体构建、重组表达载体诱导表达和重组蛋白纯化；所述重组表达载体构建为将原核表达载体PET32a与目的核苷酸片段进行重组，构成可表达氨基酸序列为SEQ ID NO:2的半滑舌鳎黑色素富集素2(MCH2)多肽的重组表达载体。所述重组表达载体，实现了半滑舌鳎MCH2多肽在原核生物体内的规模化、低成本生产，通过纯化可获得用于注射用或者饲料添加的生物功能制品。
摘要（英）：

The invention relates to a cynoglossus semilaevis MCH2 protein and an in-vitro expression preparation method and application of the cynoglossus semilaevis MCH2 protein. A nucleotide sequence of the protein is SEQ ID NO:1; the sequence of a protein amino acid is SEQ ID NO:2. The in-vitro expression preparation method of the cynoglossus semilaevis MCH2 protein comprises construction of a recombinant expression vector, induction expression of the recombinant expression vector and recombinant protein purification, wherein construction of the recombinant expression vector is construction of a prokaryotic expression vector PET32a and a target nucleotide fragment to form the recombinant expression vector capable of expressing cynoglossus semilaevis melanin concentrating hormone 2 (MCH2) polypeptide of which the amino acid sequence is SEQ ID NO:2. According to the recombinant expression vector, large-scale and low-cost production of the cynoglossus semilaevis MCH2 polypeptide in a prokaryotic organism is achieved, and a biological functional product for injection or feed addition can be obtained through purification.

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