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发明公开
CN107299105A 西瓜枯萎病菌致病性FonAGL3基因、其缺失DNA片段、缺失突变体及其应用 失效 - 权利终止

西瓜枯萎病菌致病性FonAGL3基因、其缺失DNA片段、缺失突变体及其应用
摘要：
本发明公开了一种西瓜枯萎病菌致病性基因FonAGL3、其缺失DNA片段、缺失突变体及其应用。旨在解决西瓜枯萎病生物防治的技术问题。本发明根据西瓜枯萎病菌T-DNA插入突变体库分析和筛选发现的一种源于西瓜尖孢镰刀菌的致病性基因FonAGL3，采用基因同源置换原理，用抗性基因潮霉素B（ HPH）基因DNA片段替换目的基因FonAGL3的DNA片段，通过构建该基因缺失载体及野生型菌株FON-11-06遗传转化获得FonAGL3基因缺失突变体，该FonAGL3突变体菌剂具有良好枯萎病防治效果，且环境友好，防治成本低廉。
摘要（英）：

The invention discloses a watermelon oxysporum pathogenic FonAGL3 gene as well as a deleted DNA fragment, a deletion mutant and application thereof, and aims to solve the technical problem of biological prevention and treatment on watermelon oxysporum. A pathogenic gene FonAGL3 derived from watermelon fusarium oxysporum is analyzed and screened by inserting watermelon oxysporum T-DNA into a mutant library, by utilizing the homologous gene replacement principle, DNA fragments of the target gene FonAGL3 are replaced by gene DNA fragments of a resistance gene hygromycin B (HPH), a FonAGL3 gene deletion mutant is obtained by establishing a gene deletion carrier and implementing genetic transformation of a wild strain FON-11-06, and the FonAGL3 mutant bacterium has a good wilt prevention and treatment effect and is environmental-friendly and low in prevention and treatment cost.

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