The invention provides a calling method and device for a debugging tool. The calling method comprises the steps that a calling command for an application program file of the debugging tool is received, wherein the debugging tool is a non-starting item, and the application program file is located in a file storage region of an upper computer memory; the application program file is loaded into a program running region of the upper computer memory according to the calling command; and the application program file is executed for debugging. Through the calling method and device, the problem that through existing calling methods for the debugging tool, much memory space is occupied, and consequently a vxWorks operating system is prevented from improving running efficiency is solved.