Disclosed is a double-ring sealing device for controlling cross contamination. The double-ring sealing device for controlling cross contamination comprises a flange assembly, a sealing cover assembly and a separation preventing assembly. The flange assembly comprises a connecting plate, an upper flange, a lower flange and a transfer channel. The connecting plate is arranged in a transfer connector formed between two adjacent box chambers, the upper flange and the lower flange stretch into the box chambers, and the transfer channel penetrates through the connecting plate, the upper flange and the lower flange. The sealing cover assembly comprises a sealing cover and a grabbing device. After grabbing operation is accomplished, a transfer container cover body and the sealing cover are combined to form a first closed space, and the outer surface of the transfer container cover body is located in the first closed space. The sealing cover is detachably combined with the upper flange to form a second closed space, and a transfer container body is detachably combined with the lower flange to form a third closed space. The separation preventing assembly prevents the sealing cover from being separated from the upper flange. The double-ring sealing device for controlling cross contamination has the advantages that the purpose that cross contamination of atmospheres in box bodies cannot happen in the transfer process, and the outer surfaces of product cups cannot be contaminated by the atmospheres in the box bodies on the inner side.