The invention relates to a method for detecting salmonella based on a nucleic acid chromatography biosensing technology. According to a virulence gene invA of salmonella, loop-mediated isothermal amplification primers (SEQ ID NO:1-4) are designed, and through the combination with a nano-enzyme nucleic acid test strip, the salmonella detection method based on an LAMP nano-enzyme sensor is established. The method for detecting salmonella based on the nucleic acid chromatography biosensing technology can be successfully used for distinguishing live bacterial cells from dead bacterial cells, and the lower limit of detection on salmonella can reach 10 CFU/mL.