The invention discloses a preparation method of a starch-free microcapsule starter feed for flounders, plaices, and soles. The microcapsule starter feed is prepared from the following raw materials in percentage by weight: 80 to 90% of protein source raw material, 6 to 18% of fat source raw material, 0.5 to 1% of composite vitamins, 0.5 to 1% of composite mineral matters, 0.5 to 1% of astragalus polysaccharide, and 0.5 to 1% of vine tea flavones. The microcapsule starter feed is prepared by the following steps: grinding the protein source raw material into micro powder with a size of 325 to 450 meshes through ultrafine grinding, fully mixing the micro powder with the fat source raw material, composite vitamins, composite mineral matters, astragalus polysaccharide and vine tea flavones; adding water, blending, carrying out emulsification for 10 to 20 minutes to prepare thick slurry (20-35%), carrying out homogenization under a pressure of 10-20 MPa, and finally performing spraying, drying, sieving and cooling to obtain the microcapsule starter feed. The preparation method has the advantages that no additional wall material (starch, gelatin, or the like) is needed, and the colloid of the protein source raw material wraps other raw materials to form microcapsule particles. Furthermore, the starter feed is rich in nutrients and is palatable, feed waste is avoided, and water pollution and environmental pollution are prevented.