The invention provides a preparation method of soy sauce with a healthcare function. The soy sauce comprises the steps as follows: (1) fructus momordicae and radix glycyrrhizae are crushed and mixed, then mixed with water with the weight 10-15 times that of the fructus momordicae and radix glycyrrhizae to be decocted for 1-2 h, decocted 2-3 times and filtered, filtrates are mixed and concentrated until every 1 mL contains 1 g of raw medicinal materials, and then sterilization is performed; (2) the filtrate obtained in step (1) is blended with fermented soy sauce and the soy sauce with the healthcare function is prepared. The invention further provides the soy sauce with the healthcare function and prepared with the method. The soy sauce has a good function of removing heat from the lung to relieve cough and is suitable to be eaten by people.