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发明公开
CN107384843A 少动鞘脂单胞菌基因敲除突变株及其构建方法 无效 - 驳回

少动鞘脂单胞菌基因敲除突变株及其构建方法
摘要：
本发明提供了一种少动鞘脂单胞菌基因敲除突变株及其构建方法，菌株保藏于中国典型培养物保藏中心，保藏号为CCTCC No.2016610；本发明构建了一种PHB缺失少动鞘脂单胞菌突变株，利用同源重组敲除少动鞘脂单胞菌中PHB合成途径中的乙酰乙酰辅酶A还原酶的基因，得到基因缺失突变株，从而抑制PHB的产生，提高了胶体的纯度和透明度；同时保持了较高的产胶率和产胶稳定性；从而大大提高了大规模生产去除PHB的透明型高酰结兰胶的可能性。
摘要（英）：

The invention provides a sphingomonas paucimobilis gene knockout mutant strain and a construction method thereof. The strain is collected in China Center for Type Culture Collection and has the collection number of CCTCC No. 2016610. A PHB deletion sphingomonas paucimobilis gene knockout mutant strain is constructed, and genes of acetoacetyl coenzyme A reductase in a PHB synthetic route of sphingomonas paucimobilis are knocked out by utilizing homologous recombination so as to obtain the gene deletion mutant strain, so that production of the PHB is inhibited, and the purity and transparency of colloid are improved; and meanwhile, the colloid productive rate and colloid production stability are maintained, so that the possibility of removing transparent high acyl gellan gum of the PHB in large-scale production is greatly improved.

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