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发明公开
CN107410018A 一种叶用甘薯组培苗光合自养的环境调控方法 无效 - 撤回

一种叶用甘薯组培苗光合自养的环境调控方法
摘要：
本发明是一种叶用甘薯组培苗光合自养的环境调控方法，具体步骤为：预培养：将外植体接种到MS基础培养基上，培养容器为透明、罐形玻璃瓶，放置在传统的培养架上预培养8天；环境调控培养：预培养结束后，将接种外植体的培养容器放置于环境调控系统中进行培养，先在系统内设置二氧化碳浓度为800μmol·mol-1，光合光量子通量密度为46μmol·m-2·s-1，生长2天，使其逐步适应环境；再调整二氧化碳浓度为8500μmol·mol-1，光合光量子通量密度为250μmol·m-2·s-1，继续生根培养20天，得到甘薯植株。本发明提供了一种叶用甘薯组培苗光合自养的环境调控方法，植株生长健壮，根系发达。
摘要（英）：

The invention relates to an environment regulation method for realizing photoautotrophy of a leaf sweet potato tissue culture seedling. The environment regulation method comprises the following specific steps: performing preculture: inoculating an MS basal culture medium with an explant, putting a culture vessel which is a transparent pot-shaped glass bottle on a traditional culture shelf, and performing preculture for 8 days; performing environment regulation culture: after the preculture is completed, putting the culture vessel inoculated with the explant in an environment regulation system, performing culture in the system in which the carbon dioxide concentration is 800 [mu]mol.mol<-1> and the photosynthetic photon flux density is 46 [mu]mol.m<-2>.s<-1>, and enabling the explant to grow for 2 days and gradually adapt to the environment; and regulating the carbon dioxide concentration to 8500 [mu]mol.mol<-1> and the photosynthetic photon flux density to 250 [mu]mol.m<-2>.s<-1>, and further performing rooting culture for 20 day to obtain a sweet potato plant. According to the environment regulation method for realizing photoautotrophy of the leaf sweet potato tissue culture seedling, the plant grows strongly, and the root system is developed.

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