The invention discloses a method for recovering doxycycline hydrochloride from a doxycycline hydrochloride refining mother liquid. The method comprises the steps: 1) adding sulfosalicylic acid into the doxycycline hydrochloride refining mother liquid to treat the doxycycline hydrochloride refining mother liquid, standing and crystallizing the doxycycline hydrochloride refining mother liquid, and filtering a separated solid A; 2) adding an ethanol water solution into the filtered solid A, carrying out pulping and washing, and filtering the solution to obtain a solid B; 3) adding the solid B into the ethanol water solution, dropwise adding ammonia water until the solid is dissolved to be clear, heating the solution after filtering, and filtering the solution after crystallizing to obtain a solid C; 4) adding the solid C into the ethanol water solution, dropwise adding hydrochloric acid to dissolve the solid C, adding sulfosalicylic acid, and filtering a separated solid D after crystallizing; and 5) adding the solid D into the ethanol water solution, dropwise adding ammonia water until the solid is dissolved to be clear, heating the solution after filtering, and filtering the solution after crystallizing to obtain a solid E; and 6) adding the solid E into the ethanol water solution, adding hydrochloric acid to carry out refining, and carrying out filtering after crystallizing to obtain a solid, namely doxycycline hydrochloride. Not only is the method environment-friendly, but also the recovery rate of doxycycline hydrochloride reaches 92-95%.