The invention provides a preparation method of a ceramic-substrate colorful cladding layer. The preparation method comprises the following steps: (1) grinding, polishing, washing and drying a ceramic substrate, putting the ceramic substrate into a vacuum furnace, wherein the vacuum degree inside the furnace is 0.2 * 10<-1> to 1.5 * 10<-1> Pa, and the temperature is 50 to 200 DEG C, and preheating the ceramic substrate for 0.5 to 2 min; (2) filling argon to carry out ion bombardment washing on the ceramic substrate for 2 to 10 min; (3) carrying out secondary cladding on a source electrode target material which is a titanium alloy comprising 50% to 70% of Ti, 17% to 30% of Cr, 10% to 20% of Ni, 2% to 8% of Al and 0.2% to 3% of Au in percentages by weight, introducing nitrogen, acetylene, oxygen or mixture gas thereof, and carrying out cladding of different colors of cladding layers. The invention also provides the ceramic-substrate colorful cladding layer. The cladding layer is of various colors, and is high in bonding force with the ceramic substrate, abrasive resistance, and corrosion resistance, and free of discoloring for long time.