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发明公开
CN107460250A 基于KIF14、KIF15和KIF20A基因的透明细胞肾癌诊断试剂盒及其使用方法 有权

基于KIF14、KIF15和KIF20A基因的透明细胞肾癌诊断试剂盒及其使用方法
摘要：
本发明属于检测试剂盒技术领域，具体涉及一种基于KIF14、KIF15和KIF20A基因的透明细胞肾癌诊断试剂盒及其使用方法，所述试剂盒包括扩增KIF14、KIF15和KIF20A基因的PCR引物对和扩增管家基因GAPDH的PCR引物对。本发明首次发现KIF14、KIF15和KIF20A基因在透明细胞肾癌肿瘤部位和癌旁正常组织转录表达水平存在显著差异。本试剂盒通过联合检测KIF14、KIF15和KIF20A基因转录水平的表达情况并与正常组织表达情况对比，可以快速、高效、精准的诊断透明细胞肾癌。
摘要（英）：

The invention belongs to the technical field of detection kits and particularly relates to a transparent cell kidney cancer diagnostic kit based on genes KIF14, KIF15 and KIF 20A and a use method of the diagnostic kit. The kit comprises a PCR primer pair for amplifying the genes KIF14, KIF15 and KIF 20A as well as a primer pair for amplifying a housekeeping gene GAPDH. According to the diagnostic kit, the significant differences of the genes KIF14, KIF15 and KIF 20A on the transcription expression levels of transparent cell kidney cancer tumor sites and paracancerous normal tissues are found for the first time. By jointly detecting the transcription levels of the genes KIF14, KIF15 and KIF 20A and making comparison on the transcription levels and expression conditions of the normal tissues, the transparent cell kidney cancer can be rapidly, efficiently and precisely diagnosed.

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