The invention provides an optimal selection method of a reference station. The optimal selection method of the reference station comprises the steps of S1, segmenting a target area based on the border of a coverage area of each reference station in the target area so as to obtain multiple coverage fragments, wherein the target area is the total coverage area of all the reference stations; S2, building a relation table between each reference station and the multiple coverage fragments based on the coverage relationship between each reference station and the multiple coverage fragments; and S3, judging whether the reference stations can be replaced respectively based on the relation table, and getting rid of the replaceable reference stations of the reference stations. The optimal selection method of the reference station can reduce the number of the reference stations distributed in the target area; and resources are saved. Meanwhile, due to the reduction of the number of the reference stations distributed in the target area, radio interference among the reference stations can be reduced, and normal operation of an automatic driving navigation system in the target area can be guaranteed.