The invention provides an MEH-PPV concentrated solution-based single-mode random laser generation method. The method comprises the steps of preparing an MEH-PPV concentration solution firstly; next, setting a laser, two polarization apparatuses, a circular hole diaphragm, a columnar lens, a narrow gap and MEH-PPV concentrated solution in sequence to enable the laser, the two polarization apparatuses, the diaphragm, the columnar lens and the narrow gap to be coaxial, and putting the concentrated solution in the focal point position of the columnar lens; and finally, regulating the laser to generate laser with pulse energy of 10-12[mu]J/pulse, enabling the laser to pass through the two polarization apparatuses to be intensified, and then to pass through the circular hole diaphragm to form circular light spots with uniform light intensity distribution, and focusing the circular light spots by the columnar lens to form linear light spots, adjusting the narrow gap to enable the linear light spot length to be 9-10mm, and enabling the linear light spots to excite the concentration solution to generate the single-mode random laser. The single-mode laser generation process is simple and can be repeated, and the expected single-mode random laser can be obtained; and in addition, bright single-mode random laser can be output, and the conversion efficiency can reach about 10%.