The invention discloses a multichannel microdroplet detection chip for nucleic acid detection, which is successively provided with a cover plate layer, a channel layer and a substrate layer from top to bottom, wherein the channel layer is internally provided with a plurality of independent detecting units; each detecting unit comprises a dispersion phase hole, a sample adding hole, a reaction cavity and a reaction cavity hole; the dispersion phase holes and the sample adding holes are respectively converged through a first micro-channel and a second micro-channel, then are communicated to the reaction cavity through a third micro-channel; the other end, opposite to the third micro-channel, in the reaction cavity is communicated with the reaction cavity hole through a forth micro-channel. According to the multichannel microdroplet detection chip, a chip structure of a plurality of parallel detecting units is adopted to improve the reaction flux; a buckle is used for connecting different chips, the different chips can be detected in a combining manner, the flux is flexible and adjustable, and the detection efficiency is improved; the multichannel microdroplet detection chip is simple in structure, the application cost is greatly reduced compared with the present digital PCR instrument, and can be applied to the primary clinical detection.