The invention provides a mask plate, a vapor deposition mask plate assembly, vapor deposition equipment and a manufacturing method of the mask plate. The mask plate comprises a mask plate main body, wherein the mask plate main body is provided with a shielding part and a plurality of opening parts; the mask plate main body comprises two sides facing away from each other; a magnetic layer is arranged on a reserved region on at least one side of the two sides facing away from each other. By adopting the manufacturing method, the defects of color mixing, poor uniformity and the like due to unevenness of the mask plate during vapor deposition can be overcome, and the product yield is increased.