The invention discloses a wheel type sheep embryo transplantation frame and an operation method thereof. The wheel type sheep embryo transplantation frame comprises universal wheels, a supporting column, a base plate, a limiting clamping groove, a release pedal, a head pillow, a lifting bearing, a lying bed, a telescopic rod, front leg fixing clamps, rear leg fixing clamping locks, rear leg limiting rod, rear leg limiting rods and rear leg limiting lock holes, wherein the universal wheels are fixed to the four corners of the base plate through screws and nuts respectively, the supporting column is welded to the base plate, the limiting clamping groove is stretched and folded through a joint screw, the release pedal is pulled up and released through a screw, the head pillow is slightly higher than the lying bed, the lifting bearing is used for lifting the frame to certain height, the lying bed is used for an animal to lie, the telescopic rod controls stretching of the frame by means ofthe clamping groove design, the front leg fixing clamps are welded to the head pillow end, the rear leg fixing clamping locks are welded to the other end of the frame, and the rear leg limiting rods fix sheep rear legs through the rear leg limiting lock holes. The wheel type sheep embryo transplantation frame has the advantages of being convenient to use, easy and safe to operate and suitable forapplication and popularization.