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发明授权
CN107630014B 一种基于大数据的直缘乌头DNA条形码及直缘乌头的鉴定方法 有权 转让

一种基于大数据的直缘乌头DNA条形码及直缘乌头的鉴定方法
摘要：
本发明公开了一种基于大数据的直缘乌头DNA条形码及直缘乌头的鉴定方法，属于分子鉴定技术领域。通过广泛调查直缘乌头的野外分布区、主要栽培区、野外同域分布物种以及近缘药用物种的资料信息，然后尽可能的进行多个地方采样，构建样品的DNA条形码大数据库，比较并发现直缘乌头特有的序列信息位点，进一步获得其特有的可用于鉴定的DNA条形码。所述DNA条形码为由引物序列SEQ ID NO.1和SEQ ID NO.2扩增得到的总长为443bp的序列，其中第106‑112bp为碱基ACTAAGA，第201‑210bp为碱基CCTATCTATA。利用该DNA条形码将大大有利于药用植物直缘乌头的快速鉴定。
摘要（英）：

The invention discloses a big data based identification method for an aconitum transsectum DNA barcode and aconitum transsectum, belonging to the technical field of molecular identification. By extensively investigating data information of field distribution areas, main cultivation areas, field sympatric species and closely related medicinal species, and then sampling in as many places as possibleto create a big database for DNA barcodes of samples, sequence information sites unique to aconitum transsectum are compared and found, and then the DNA barcode which can be used for identification unique to the aconitum transsectum is further obtained. The DNA barcode is a sequence with a total length of 443bp obtained by amplification of primer sequences SEQ ID NO. 1 and SEQ ID NO. 2, wherein 106-112bp is basic ACTAAGA, and 201-210bp is basic CCTATCTATA. The utilization of the DNA barcode can be greatly beneficial to the rapid identification of the medicinal plant aconitum transsectum.

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