The invention discloses a tripterygium wilfordii extractive, preparation method thereof and the application thereof to the preparation of eye drops. The preparation method comprises the following steps: extracting tripterygium wilfordii medical material decoction pieces with 70 to 95 percent ethanol, filtering, combining filtrate, recycling ethanol under reduced pressure till no alcohol taste exists, continuously concentrating the filtrate to form extract with the relative density of 1.15 to 1.35, performing adsorption with neutral aluminum oxide, drying under reduced pressure and crushing, soas to obtain a tripterygium wilfordii extractive neutral aluminum oxide mixed sample; adding trichloromethane to the tripterygium wilfordii extractive neutral aluminum oxide mixed sample to perform extraction, filtering, taking filtrate, recycling the trichloromethane, and drying, so as to obtain the tripterygium wilfordii extractive. The tripterygium wilfordii extractive overcomes the defect ofhigh toxicity when the tripterygium wilfordii extractive is orally taken, is applied to the preparation of eye drops, has the advantages of safety, effectiveness and stability, the eye drops preparedthrough the ripterygium wilfordii extractive have excellent curative effect on xerophthalmia, and the tripterygium wilfordii extractive has a good application prospect and market value.