The invention provides a preparation method of actinidia chinensis ferment jelly. The preparation method comprises the following steps of: carefully choosing actinidia chinensis, performing peeling, removing the kernel from the actinidia chinensis, performing cleaning treatment, performing dicing and mashing, adding water, performing uniform stirring, performing homogenization, performing centrifugation, and taking supernatant; performing subpackaging, adding white granulated sugar, performing moist-heat sterilization, performing cooling and inoculation with a seed solution (lactobacillus plantarum, lactobacillus acidophilus, bifidobacterium and streptococcus thermophilus) at sterile working environment, performing uniform shaking, and performing fermentation at 25 DEG C- 37 DEG C for 28-48h so as to obtain actinidia chinensis ferment fermentation liquor; then adding a certain amount of jelly liquor and performing compounding (wherein the preparation method of the jelly liquor comprises the steps of under the condition of stirring, pouring pectin, citric acid, white granulated sugar and the like into preheated pure water about 80 DEG C, continuing performing stirring so as to allove the pectin, the citric acid, the white granulated sugar and the like to dissolve uniformly, performing binding, performing sealing, performing moist-heat sterilization, and performing cooling to 70DEG C so as to obtain the jelly liquor for standby application), performing homogenizing and filling, performing sealing and performing pasteurization; and performing standing under the condition of 8DEG C, and performing coagulate freezing so as to obtain probiotic actinidia chinensis ferment jelly. According to the actinidia chinensis ferment jelly disclosed by the invention, the active ingredients of the actinidia chinensis are increased, and besides, the ferment is convenient to eat.