The invention provides a poultry oil emulsion inactivated vaccine adjuvant and a preparation method of a corresponding vaccine of the adjuvant. Through specific screening and combination, the functions can be better exerted, so that all ingredients make good for deficiency; the poultry oil emulsion inactivated vaccine adjuvant obtained through preparation can non-specifically and specifically enhance the vaccine immunity effect, promote the inactivated vaccine absorption and retard the antibody attenuation. The preparation method of the novel poultry oil emulsion inactivated vaccine adjuvant is simple; the implementation is easy; the cost is low; the performance is stable; safety and high efficiency are realized; the applicability is wide; the large-scale production and popularization areconvenient; the defects of poor absorption performance, low antibody valence and poor immune effect of the traditional vaccine adjuvant are overcome.