The invention relates to a bait agent for killing subterranean termites in the whole nest and a production method of the bait agent. The bait agent is prepared from the following components in parts by weight: 40 to 50 parts of graphene oxide, 5 to 10 parts of piperidine, 1 to 2 parts of benzophenone, 4 to 5 parts of potassium sorbate, 2 to 4 parts of chitosan and 1 to 1.5 parts of hydroxymethyl cellulose. According to the bait agent for killing the subterranean termites in the whole nest, disclosed by the invention, the knock-down time range of 50 percent of the termites is 4 to 48 hours andthe virus transmission effect is better; particularly, the traditional moderate toxicity is transformed into chronic toxicity, so that the knock-down effect on the termites is relatively small, the knock-down time is relatively long and the transmission of the termites to the drug toxicity is facilitated.