The invention discloses antibacterial cool functional fabric and a preparation method thereof. Antibacterial cool superfine polyester filaments serving as raw materials are kitted into weft-knitted double-sided jacquard fabric through a circular weft knitting machine; the superfine polyester fibers are prepared from, by weight, 1-20% of silver-based antibacterial master batches, 5-20% of thermally-conductive master batches and 60-91% of polyester chips, the sections of the fibers are special-shaped, and the fiber fineness is 0.45 D. Since the antibacterial cool functional fabric achieves the cool effect and the antibacterial function through the fibers, the fabric still has excellent performance of moisture absorption, quick drying, rapid cooling, bacterium resistance and sterilization after extreme friction and repeated washing operation.