The invention discloses a device for automatic regulation of an ultraviolet laser beam position and a regulation method of the device. The device includes a quasimolecule ultraviolet laser device, a first reflector, an electronic control translation platform, a second reflection lens, a cylindrical mirror, a phase mask board, an optical fiber and a white screen. The quasimolecule ultraviolet laserdevice emits ultraviolet light beams which is then reflected to the second reflection lens by the first reflection lens, reflected to the cylindrical mirror by the second reflection lens, focused tothe phase mask board through the cylindrical mirror and casts into the optical fiber, so that ultraviolet light beams achieve filament diffraction through the optical fiber and strips are formed on the white screen. A CCD camera collects information about whether the strips on the white screen are symmetric or not and the strip information collected by the CCD camera is sent to a computer. If thestrips are not symmetric, the computer feeds a signal back to piezoelectric ceramics. The piezoelectric ceramics receives the signal and regulates the second reflection lens, so that the ultraviolet beams can cast to the optical fiber exactly and the central axis of the strips on the white screen can be symmetric in the vertical direction.