The invention discloses a green accurate preserving and fresh-keeping method for blueberries and an application thereof. The method comprises the following steps: spraying treatment is carried out forblueberries with a before-harvest biological antistaling agent, fruits are nondestructively graded according to sizes, the fruits are harvested and placed in a precooling refrigerator in 3-5 hours, rapid precooling is carried out for 6-10 hours, precooling is carried out for 4-8 hours at 0+/-0.5 DEG C, and then an antifog fresh-keeping bag whose thickness is 20-35[mu]m for slightly controlled atmosphere is used as an outer lining of a small basket for sealing; low temperature domestication is carried out at -1.6 to -1.2 DEG C for 6-8 hours, storage is carried out for 60-70 days with accuratetemperature control at -0.8 to -0.5 DEG C or -0.5 to -0.2 DEG C, the temperature of the refrigerator is adjusted to 0-0.5 DEG C, awaking time is 48-72 hours, awaking is carried out again at 10+/-0.5 DEG C for 24-36 hours, external packing is removed in order to carry out high-concentration 1-MCP fumigating treatment, and commercial package of blueberries is carried out. Combination of technology and technological parameter is used, in order to substantially maintain original quality of blueberries and improve quality of blueberries.