The invention discloses a breeding method of a hybridization strain of top-rot-resisting sugarcanes. The method comprises the following steps: heeling sugarcane seedlings into plugs; then spraying a prepared sugarcane top rot pathogenic bacterium spore suspension solution on interior leaves of the sugarcane seedlings after 7 to 8 true leaves grow out; establishing a small arch shed and keeping humidity and temperature in the shed; after 3 days, uncovering a thin film on the small arch shed and investigating a morbidity situation after 15 days; screening a new sugarcane top rot-resisting variety. According to the breeding method disclosed by the invention, by inoculating the top rot pathogenic bacterium, the effect of forcing the sugarcane seedlings is achieved, infected seedling single plants and a large number of infected hybridized combinations are eliminated, so that top-rot-resisting hybridized combinations and single plants are screened primarily; a traditional sugarcane seedlingtop-rot-resisting breeding method is changed by the method; the breeding method has the advantages of remarkable morbidity, strong operability and capabilities of shortening the breeding age limit ofbreeding the new sugarcane top-rot-resisting variety and improving the breeding efficiency.