The invention relates to a production method using bacillus to produce ZY peptide, and belongs to the technical field of biology. The production method comprises the following steps of using the bacillus as a production bacteria strain; preparing materials, fermenting, placing into a tank, centrifuging, pretreating, separating by a membrane, spraying, and drying, so as to obtain a finished productof the ZY peptide. The production method has the advantages that by performing the three steps of centrifuging, pretreatment and membrane separating, the bacterial cell and impure protein can be furthest removed, and a pure product of the ZY peptide is obtained; the heat-resistant property and stability of short peptide of the ZY peptide are utilized, the spraying and drying technique is utilized, and maltodextrin is used as an excipient, so as to obtain the finished product of ZY peptide with good dissolvability and high stability; the finished product can be directly used as a feed additiveto be added into livestock and aquaculture feed; activity of the separated and purified ZY peptide is high, the cost is low, the method is simple, and the activity and recycling rate of the ZY peptide are high.