The invention discloses a rotary low-impact self-separation pressing and releasing device. The upper end of a compressing rod compresses a separation spring onto an upper end cover through a compressing cap, the compressing rod sequentially penetrates through the upper end cover, an upper base, a limiting block and a rotating lock petal, the lower end of the compressing rod is fixedly connected with a separation nut, and the separation nut is fixedly connected with a lower base; a compressing nut is fixed onto the compressing rod and used for limiting rotating motion of the locking petal through a limiting block; an unlocking spring is installed between a stop dog of the rotating lock petal and a stop dog of the upper base, when the device is locked, the separation spring and the unlockingspring are in a pre-compressed state, and tapered teeth at the bottom of the rotating lock petal is occlusive in an over against mode. By means of the rotary low-impact self-separation pressing and releasing device, connected objects at the two ends can be connected and separated and do not move relatively during separation.