The invention discloses spore fungus powder. The spore fungus powder is prepared from the following components by weight percent: 31.1 to 70 percent of bacillus subtilis, 20.8 to 50 percent of bacillus licheniformis, 4 to 16 percent of bacillus megaterium, 2 to 5 percent of vitamin C, and 0.03 to 0.1 percent of high-activity enzyme. The spore fungus powder obtained by the invention can rapidly decompose and convert residual bait, manure and sludge in the water, can improve the water environment and increase the dissolved oxygen, can control the growth of various weeds, can prevent the growth of blue-green algae, euglena, moss, filamentous green algae and ceratodictyon spongiosum, so that the harmful bacteria in the water can be greatly reduced, a purifying and detoxifying effect can be achieved, the water quality can be effectively improved, the deterioration of the water environment and the diseases such as virus diseases, bacterial diseases, secretly-dying disease and the like can beprevented, a culture object can be prevented from having less disease or having diseases, and the growth of the culture object can be facilitated.