The invention provides a non-refrigeration photoelectric detector relative spectrum response temperature characteristic calibration method. The monochromatic light is collected in a vacuum temperaturecontrol system by an off-axis optical system at the output of a monochromator and is received by a standard detector in the vacuum temperature control system. An output signal of the detector is amplified and then sent to a data acquisition system, and change of the output wavelength of the monochromator is controlled by the controller. During measurement, the standard detector is firstly calibrated in the vacuum temperature control system, then the standard detector is removed through a precise displacement turning table, a measured detector is turned to the same position, after the vacuum system reaches the set state, a thermostat is set to be in different temperatures, after temperature stabilization, non-refrigeration photoelectric detector relative spectrum response measurement underdifferent temperatures is carried out. The method is advantaged in that not only can the monochromatic light be prevented from irradiating onto the detected infrared detector, but also interference because of frosting and steam generation on the surface of a non-refrigeration photoelectric detector caused by increase or decrease of the surrounding temperature can be avoided.