The invention relates to a culture medium which can quickly and effectively induce reprogramming of mice somatic cells into pluripotent stem cells and has no animal exogenous property. The induction culture medium is composed of a DMEM base culture medium, NEAA, KSR, LIF and a variety of small molecules and inorganic salts; the culture medium has clear components and has no animal exogenous pollution. The induction culture medium has the advantages of high induction efficiency, short induction time and the like, and is of great significance for development of obtaining of a large number of induced pluripotent stem cells and promotion of clinical application of the stem cells.