The invention discloses a fermentation type compound preparation having effects of relieving weariness and regulating immunity and a preparation method of the fermentation type compound preparation. In the fermentation process of medical and edible fungi of Chinese caterpillar funguses, glossy ganoderma, yeast, aspergillus and the like, a rhizoma polygonati extract, a Chinese wolfberry fruit extract, a longan extract, a red jujube extract and a concha meretricis seu cyclinae extract are added, and after fermentation, homogenizing and spray drying are performed, so that the fermentation type compound preparation is obtained, and product forms of chewable tablets, granules, capsules and the like can be further developed. The compound preparation is fragrant and sweet in smell, good in mouthfeel and high in activity, and has favorable effects of relieving weariness and regulating the immunity.